Light Conference 2020国际学术会议
Rising Stars of Light奖
为激励青年学者发表重大创新结果，会议特设“Rising Stars of Light”奖，其中：一等奖1名（奖金2万元人民币），二等奖2名（奖金1万元人民币），三等奖3名（奖金5000元人民币）。其学术成果必须是未发表、未投稿且与光学研究相关的原创性工作。具体评选办法及获奖成果报告交流方式等，请参见会议官方网站。
Stem-cell therapy is showing great potential in regenerative medicine because of their inherent ability to self-renew and differentiate. Biochemical and genetic methods and 3D materials/structures have been developed and making great progresses on controlling the development and differentiation of stem cells. One of the major remaining concerns is the invasion of those technologies. In this study, we demonstrate an all-optical and noninvasive approach to precisely induce the differentiation of mesenchymal stem cells (MSCs) and cerebellar granule neuron progenitor (GNP) cells. We show that the single-time fast-flash of photostimulation by a tightly-focused femtosecond laser, without any optogenetics, can activate endogenous signaling pathways for stem-cell differentiation directly by multiphoton excitation. The significant upregulation of differentiation regulator RUNX2 and Osterix in MSCs 7 days after photostimulation indicates the osteogenic differentiation of MSCs. The differentiation was finally confirmed by Alizarin Red staining 28 days after photostimulation. The differentiation of GNP cells in vitro and in vivo can also be induced by this method. This noninvasive optical technology hence provides an encouraging advance in activation of signaling pathways in cells and alternative to classic biochemical methods for stem-cell differentiation. This result also provides an optical choice with promising potential for clinical regenerative applications.