Light Conference 2019 International Academic Conference
Submissions to ‘Rising Star of Light’
'Rising Stars of Light' Awards
To encourage young scholars to publish their innovative work, we'd like to set up an award, 'Rising Stars of Light'. The academic achievements of all the award recipients must be original and unpublished in optics: one first-prize (a prize of ¥ 20000), two second-prizes (a prize of ¥ 10000) and three third-prizes (a prize of ¥ 5000) will be selected on site. Please see official website for more specific selection rules and procedures. Language: English.
Submission deadline:April 10, 2019
Optical regulation of stem-cell differentiation by femtosecond-laser photostimulation Author：Wanyi Tang DownloadStem-cell therapy is showing great potential in regenerative medicine because of their inherent ability to self-renew and differentiate. Biochemical and genetic methods and 3D materials/structures have been developed and making great progresses on controlling the development and differentiation of stem cells. One of the major remaining concerns is the invasion of those technologies. In this study, we demonstrate an all-optical and noninvasive approach to precisely induce the differentiation of mesenchymal stem cells (MSCs) and cerebellar granule neuron progenitor (GNP) cells. We show that the single-time fast-flash of photostimulation by a tightly-focused femtosecond laser, without any optogenetics, can activate endogenous signaling pathways for stem-cell differentiation directly by multiphoton excitation. The significant upregulation of differentiation regulator RUNX2 and Osterix in MSCs 7 days after photostimulation indicates the osteogenic differentiation of MSCs. The differentiation was finally confirmed by Alizarin Red staining 28 days after photostimulation. The differentiation of GNP cells in vitro and in vivo can also be induced by this method. This noninvasive optical technology hence provides an encouraging advance in activation of signaling pathways in cells and alternative to classic biochemical methods for stem-cell differentiation. This result also provides an optical choice with promising potential for clinical regenerative applications.